Abstract:

Objective To investigate whether lactic acid(LA),which was extracted from the culture supernatant of Lactobacillus,could inhibit lipopolysaccharide(LPS)-induced translocation of NF-κB p65 from cytoplasm to nucleus and transcription of NF-κB p65 and cyclooxygenase 2(COX-2). Methods Rat intestinal mucosa microvascular endothelial cells(RIMMVEs) were cultured in vitro and were divided into 4 groups:control group,LPS group,LA pretreated group and NF-κB inhibitor (pyrrolidinecarbodithioate,PDTC) pretreated group.The protein of NF-κB p65 in cytoplasm and in nucleus was detected by Western blotting analysis after RIMMVEs were treated with LPS for 30 minutes.The mRNA of NF-κB p65 and COX-2 was detected by real time quantity-PCR after RIMMVEs were treated with LPS for 9 hours. Results In a short time(treated with LPS for 30 minutes),the protein expression of NF-κB p65 had no notable difference but the ratios of p65 in nuclei of LA and PDTC pretreated group were notably lower than that of LPS group.After a longer time(treated with LPS for 9 hours),the mRNA expression of NF-κB p65 and COX-2 were notably lower than LPS group. Conclusion LA has the effects like NF-κB inhibitor,notably inhibiting LPS-induced translocation of NF-κB p65 from cytoplasm to nucleus and transcription of NF-κB p65 and COX-2.By this way,LA inhibits inflammatory response.

Key words: Lactic acid , Lipopolysaccharide , Nuclear factor-κB , Cyclooxygenase 2 , Mucosa microvascular endothelial cells, Western blotting, Real time quantity-PCR , Rat